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Protein concentration was quantified using a BCA protein assay Kit (Beyotime, Shanghai) and then equal amounts (100 g) of total proteins were loaded on a polyacrylamide SDSPAGE gel

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Viral internalisation is through endocytosis of the bound virion in a clathrin-coated endosome, and fusion of viral and endosomal membranes and that the nucleocapsid is released into the cytoplasm

For GLP-1 RAs, semaglutide was notably associated with a significant risk of biliary tract disorders (n = 188) and infectious biliary disorders (n = 196)

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